GuideScan software for improved single and paired CRISPR guide RNA design


We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.

Nature Biotechnology
Yuri Pritykin
Yuri Pritykin
Assistant Professor of Computer Science and Genomics